My First Experiment Journey

In my used to know, Fluctuating Asymmetry (FA) is right and left size differences of organism. Lens (1999) was studied that measurements of FA can provide a sensitive early warning system for monitoring stress effects in highly threatened ecosystems.
I have been done some work for my experiment discussed about FA wing size of Eriborus argenteopilosus Cameron (Hymenoptera: Ichneumonidae) from cabbage field and reared from caterpillar Crocidolomia pavonana (Lepidoptera: Pyralidae).
Eriborus argenteopilosus is one of parasitoids usually parasitizes effectivelly some of cabbage and corn caterpillar such C. pavonana, Spodoptera litura, and Helicoverpa armigera in the field.
I used TPSdig 2 ( Rohlf 2004) program to measure wing size and FA of parasitoid.
I digitized landmark in each dot wing venation BY TPSdig 2 program.

To get the finally FA size, I used formula :

Centroid x = ∑ (x₁-x_n) / 13 ; Centroid y = ∑ (y₁-y_n) / 13

Explanation :

x₁ = first size dot from X.
x_n = n size dot from X.
y₁ = first size dot from Y.
y_n = Nn size dot from Y.
n = number all of dot.

Distance n = SQRT [ (x_n - centroid x)² + (y_n-centroid y)² ]

Explanation :

x_n = size of n dot from X.

y_n = size of n dot from Y.

Finally, we can get FA :

FA = │ wide of right wing-wide of left wing │